- Cells and target material in suspension
- High-speed cell deformation (squeezing)
- Temporary disruption of the cell membrane
- Target material enters the cell
- Membrane reseals
How does it COMPARE TO ALTERNATIVE TECHNOLOGIES?
Note: The comparison was performed by delivering transcription factors (proteins) for cell reprogramming.
Note: Results vary per cell type. Contact SQZ staff for more details.
ENABLES NEW APPLICATIONS
What do we use to squeeze the cells? 3 COMPONENTS
The CellSqueeze chip is mounted onto an interface with reservoirs to hold cells before and after squeezing.
Microfluidic channels with cell deforming features are etched onto silicon chips and sealed with a glass layer. These disposable chips are the functional core of our platform.
The cells move through the CellSqueeze chip under positive pressure. Our control system ensures uniform flow through the microfluidic channels.
NOT YOUR ONE SIZE FITS ALL APPROACH
Different cell sizes require different constriction geometries. A fibroblast, for example, requires a wider constriction than a naïve T cell. We have 20+ CellSqueeze chip designs with different constriction geometries.
Did you know? Our current designs can treat > 1,000,000 cells/s
OUR WORK HAS BEEN PUBLISHED IN PEER-REVIEWED JOURNALS
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