The importance of intracellular delivery

CELLS ARE THE MOST BASIC UNITS OF THE HUMAN BODY

Most diseases act at the cellular level.

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CURRENT METHODS FACE

Not applicable to many cells and molecules

 

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TWO MAJOR CHALLENGES

High toxicity and off-target effects

 

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CURRENT METHODS FACE TWO MAJOR CHALLENGES

Not applicable to many cells and molecules

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High toxicity and off-target effects

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BUILDING A TEAM

The inventors – Prof. Robert Langer, Prof. Klavs Jensen and Dr. Armon Sharei – recognized these challenges and sought to develop a more effective delivery technology.

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THE SOLUTION: CELLSQUEEZE

The exploratory work led to the development of the CellSqueeze platform. This technology has demonstrated enabling potential in applications ranging from cell therapy to fundamental studies of disease mechanisms.

HOW IT WORKS

In this process, cells are suspended in the desired buffer and are flown through a CellSqueeze chip. The chip contains microfluidic channels that rapidly deform the target cells to induce temporary disruption of the membrane. The delivery material can then enter the cell through the membrane disruptions before they reseal. Our current designs operate at throughputs of over 1,000,000 cells/s and delivery materials are selected to impart a variety of functions on the target cells. This technology thus provides a robust platform for engineering a diversity of cell functions for therapeutic applications.

SQZ BIOTECH

Through internal research programs and external partnerships, SQZ Biotech is leading a revolution in scientists’ approach to disease research and clinical therapies, developing novel methods to engineer cell function and harnessing the power of a patient’s own cells to combat disease more effectively across a broad range of indications.

How does it COMPARE TO ALTERNATIVE TECHNOLOGIES?

PNAS, Sharei et al., 2013

PLOS ONE, Sharei et al., 2015

PUBLICATIONS IN PEER-REVIEWED JOURNALS

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